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2-DE pattern (the master gel from nine gels) of germinated Pt race-1 urediniospores on 0.5% agarose for 24 h at 20 °C. The image was created by <t>Proteomescan</t> software. Gel dimensions: 24 × 20 cm. Proteins were separated with a pH 4–7 gradient in the 1st dimension, and on a 10–20% gradient SDS-PAGE in the 2nd dimension.
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2-DE pattern (the master gel from nine gels) of germinated Pt race-1 urediniospores on 0.5% agarose for 24 h at 20 °C. The image was created by <t>Proteomescan</t> software. Gel dimensions: 24 × 20 cm. Proteins were separated with a pH 4–7 gradient in the 1st dimension, and on a 10–20% gradient SDS-PAGE in the 2nd dimension.
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2-DE pattern (the master gel from nine gels) of germinated Pt race-1 urediniospores on 0.5% agarose for 24 h at 20 °C. The image was created by <t>Proteomescan</t> software. Gel dimensions: 24 × 20 cm. Proteins were separated with a pH 4–7 gradient in the 1st dimension, and on a 10–20% gradient SDS-PAGE in the 2nd dimension.
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2-DE pattern (the master gel from nine gels) of germinated Pt race-1 urediniospores on 0.5% agarose for 24 h at 20 °C. The image was created by <t>Proteomescan</t> software. Gel dimensions: 24 × 20 cm. Proteins were separated with a pH 4–7 gradient in the 1st dimension, and on a 10–20% gradient SDS-PAGE in the 2nd dimension.
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2-DE pattern (the master gel from nine gels) of germinated Pt race-1 urediniospores on 0.5% agarose for 24 h at 20 °C. The image was created by <t>Proteomescan</t> software. Gel dimensions: 24 × 20 cm. Proteins were separated with a pH 4–7 gradient in the 1st dimension, and on a 10–20% gradient SDS-PAGE in the 2nd dimension.
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Image Search Results


2-DE pattern (the master gel from nine gels) of germinated Pt race-1 urediniospores on 0.5% agarose for 24 h at 20 °C. The image was created by Proteomescan software. Gel dimensions: 24 × 20 cm. Proteins were separated with a pH 4–7 gradient in the 1st dimension, and on a 10–20% gradient SDS-PAGE in the 2nd dimension.

Journal: Scientific Reports

Article Title: The proteome study of germinated Puccinia triticina urediniospores reveals a novel effector protein required for virulence

doi: 10.1038/s41598-026-44996-2

Figure Lengend Snippet: 2-DE pattern (the master gel from nine gels) of germinated Pt race-1 urediniospores on 0.5% agarose for 24 h at 20 °C. The image was created by Proteomescan software. Gel dimensions: 24 × 20 cm. Proteins were separated with a pH 4–7 gradient in the 1st dimension, and on a 10–20% gradient SDS-PAGE in the 2nd dimension.

Article Snippet: Gels were stained with colloidal coomassie brilliant blue (CBB) and digitalized by Syngene Proteomescan Pro 2D Gel Imaging Systems at 800 dpi, and Dymension (Syngene) was used for image analysis.

Techniques: Software, SDS Page